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The Pure Culture of E. Coli Was Inoculated in the Kligler Iron Agar. The Possible Results of E. Coli Growth Are Which of the Following:

Вопрос

The pure culture of E. coli was inoculated in the Kligler iron agar. The possible results of E. coli growth are which of the following: 1. yellow slant 2. gas bubbles in the butt 3. yellow butt 4. black dots in the butt

Решения

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Ответ

The correct answer is 1, 2, and 4.Explanation:1. Yellow slant: E. coli ferments glucose and lactose, producing acid that lowers the pH of the medium. The yellow color indicates acid production.2. Gas bubbles in the butt: E. coli produces gas as a byproduct of fermentation.4. Black dots in the butt: E. coli reduces sulfur compounds, forming hydrogen sulfide (H2S), which reacts with iron salts to produceitates.Option 3 (yellow butt) is incorrect because E. coli does not ferment lactose to acid, so the butt should remain red.