3. Some enzymes are used in the clinical lab to diagnose diseases.There are two specific markers for enzymes in heart:lactute debydrogenase (LDH) and creating kinase (CK). Both of these enzymes are oligomeric, form several isoenzymes. The enzymes catalyze the following reactions: (N_(2)N-CO_(3))/(H_(2)N)-CON_(2) H_(2)gt N-C-N-CH_(2)-COOH+ADP therefore O_(2) H_(3)C-COOHxrightarrow (LDH)C-CH-COOH 1. Name the classes of these enzymes. 2. What coenzyme reguires LDH? Write the formula of the vitamine; coenzyme is synthetized from. 2. Give the definition of the term" isoenzyme" and name types of isoenzyme and proteins appeared in the blood in myocardial infurction.

1. The classes of these enzymes are:<br />- Lactate dehydrogenase (LDH)<br />- Creatine kinase (CK)<br /><br />2. The coenzyme required by LDH is NAD+ (Nicotinamide adenine dinucleotide). The vitamin synthesized from NAD+ is niacin (Vitamin B3).<br /><br />3. Isoenzymes, also known as isozymes, are different forms of the same enzyme that catalyze the same reaction but have different amino acid sequences and structural properties. They are typically found in different tissues or cell types and can have different kinetic properties or regulatory mechanisms.<br /><br />In the case of lactate dehydrogenase (LDH) and creatine kinase (CK), they exist as multiple isoenzymes that are specific to different tissues or cell types. For example, LDH has five isoenzymes (LDH1-5) that are found in different tissues such as the heart, liver, and muscles. Similarly, CK has three main isoenzymes (CK-MM, CK-MB, and CK-BB) that are found in different tissues such as the muscles, heart, and brain.<br /><br />In the context of myocardial infarction (heart attack), the types of isoenzymes and proteins that appear in the blood include:<br />- CK-MB: This isoenzyme of creatine kinase is specific to the heart muscle and is used as a diagnostic marker for myocardial infarction.<br />- Troponin: This is a complex of three regulatory proteins (troponin C, troponin I, and troponin T) that are found in skeletal and cardiac muscle fibers. The presence of cardiac-specific troponin in the blood is a highly specific marker for myocardial infarction.