7. Pyruvate kinase, a regulatory enzyme for glycolysis , is activated(indicate one or more answers): insulin ATP fructose -1,6 -diphosphate fructose-1-phosphate adrenaline

The correct answers are:<br /><br />- Fructose-1,6-diphosphate<br />- ATP<br /><br />Explanation:<br />Pyruvate kinase is a key regulatory enzyme in glycolysis, and its activity is influenced by several factors. Among the options provided:<br /><br />1. **Fructose-1,6-diphosphate**: This is an intermediate in glycolysis and activates pyruvate kinase. It indicates that the glycolytic pathway is functioning properly and that there is a sufficient supply of glucose and ATP.<br /><br />2. **ATP**: High levels of ATP inhibit pyruvate kinase. When ATP levels are high, it signals that there is enough energy in the cell, and glycolysis should be slowed down. Conversely, low levels of ATP activate pyruvate kinase to produce more ATP.<br /><br />The other options, insulin and adrenaline, do not directly activate pyruvate kinase. Insulin generally promotes glycolysis and glucose uptake, which indirectly supports the activity of pyruvate kinase. Adrenaline, on the other hand, typically promotes glycogenolysis and gluconeogenesis, which are processes that generate glucose from non-carbohydrate sources, and it does not directly activate pyruvate kinase.<br /><br />Fructose-1-phosphate is an intermediate in the metabolism of fructose but does not play a direct role in the regulation of pyruvate kinase in glycolysis.